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NEW PROGRAMME – Advanced Programme in Recombinant DNA Technology

NEW PROGRAMME – Advanced Programme in Recombinant DNA Technology

Start:
April 1, 2018
End:
April 30, 2018
Cost:
9000 + GST

• Introduction to Recombinant DNA Technology.
• Bacterial Culture Techniques.
• Isolation of Plasmid DNA.
• Agarose Gel Electrophoresis.
• DNA Restriction Analysis : Effects of DNA Methylation on Restriction.
• Rapid colony transformation of E.coli with Plasmid DNA.
• Southern Blotting Techniques.
• Purification and Identification of Plasmid DNA.
• Recombination of Antibiotic Resistance Genes.
• Transformation of E.coli with Recombinant DNA.
• SDS PAGE.
• Replica Plating to Identify Mixed E.coli Populations.
• Purification and Identification of Recombinant DNA
• Protein purification, quantification and characterization-Electrophoresis.
• PCR.
• GEL Documentation & Photography.