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Training on Molecular Biology

Training on Molecular Biology

Start:
May 3, 2019 4:00 pm
End:
May 30, 2019 4:00 pm

• THEORY – Introduction to RDT and Molecular Biology
Southern & Northern Blotting Techniques
Gene Cloning & Expression
Different Gene Delivery Methods
• PRACTICAL- Media preparation
Isolation of Plasmid DNA (Classical method and kit based)
Isolation of Bacterial Genomic DNA (Classical method)
Isolation of Plant DNA – Cetyl trimethylammonium bromide (CTAB) mtd.
Bacterial Transformation (Classical method)
Agarose Gel Electrophoresis
Qualitative and Quantitative estimation of DNA and RNA spectrophotometrically
Gel documentation and band intensity desitometry
Restriction digestion of DNA
Isolation of Genomic RNA