« View all training
New Programme – Hands on Training on Molecular Biology

New Programme – Hands on Training on Molecular Biology

January 1, 2019 10:00 am
January 30, 2019 5:00 pm
Contact Admission In Charge
SHRM Biotech Institute
Google Map
Humaipur, Kolkata, WB, India, 700155

The Programme would include the following:-

• THEORY – Introduction to RDT and Molecular Biology including Southern and Northern blotting
Gene Cloning & Expression
Different Gene Delivery Methods
• PRACTICAL- Media preparation
Isolation of Plasmid DNA (Classical method Or kit based)
Isolation of Bacterial Genomic DNA (Classical method)
Isolation of Plant DNA – Cetyl trimethylammonium bromide (CTAB) method
Bacterial Transformation (Classical method)
Agarose Gel Electrophoresis
PCR Amplification
Qualitative and Quantitative estimation of DNA and RNA spectrophotometrically
Gel documentation and band intensity desitometry
Restriction digestion of DNA (Kit based)
Isolation of Genomic RNA