1. Plasmid Isolation.
2. DNA isolation from microbes and plant sample.
3. Agarose Gel Electrophoresis.
4. RNA Isolation.
5. RNA Gel Electrophoresis.
6. Restriction Digesstion.
7. Qualitative & Quantitative analysis of Nucleic Acid.
8. Tm of DNA Melting point of DNA.
9. Blotting Techniques (Transfer of DNA & RNA from Gel to membrane).
10. Polymerase Chain Reaction (PCR)
11. Competent Cell Preparation.
12. cDNA Isolation and cloning.
13. cDNA Ligation.
14. Transformation.
15. Blue-White selection and Colony PCR.

Eligibility Criteria :
The candidates who have completed their B.Sc (Biotech, zoology, microbiology, environment science), B.Tech (Biotech), M.Sc., M.Tech, M.Phil and Ph.D in Biotechnology / Molecular Biology / Microbiology / Biochemistry / Other Life Sciences streams from a recognized institute. MBBS, BDS student also can join.

Selection Criteria :
Selection of the candidates will be based on “First Come First Serve Basis”.

Certification :
Industrial Training Certificate will be provided to the candidates who will successfully complete the Training.

Contents :
• Hands-on Modern Laboratory Techniques.
• Lectures on Lab Techniques.
• Lectures on recent topics.
• Guest lectures from esteemed institute.

Duration :
7 Days (Seven Days).

Fee Structure :
Rs.6,000/- (Rupees Six Thousand Only)
Registration Fee :
Rs.500/- (Rupees Five Hundred Only)
Total Cost – Rs 6,500.

Future Assistance to Trainees :
• Every Trainee will be assisted to develop their resume in professional manner to improve the chance of getting interview calls.
• Every Trainee will be provided hand out on “How To Face Interview”.
• Trainees will be assisted for placement by providing them listed Vacancies of R&D Companies and institutions, as when the company receives the same inquiry.

1) Introduction to Microbiology
2) Media Preparation and Sterilization
3) Serial Dilution and Pure Culture Technique
4) Different Staining Techniques
5) Biochemical Test – Catalase Test
6) IMVIC Test
7) Preservation of Wild Type and Recombimant Culture
Effect of Innoculam Size on Growth
9) Mutagenic Treatment of Bacterial Culture
10) Antibiotic Sensitivity Test for Bacterial Culture
11) Quality Control of Milk
12) Replica Plating
13) Water Analysis – MPN
14) Screening of Industrial Microbes – Caesein Hydrolysis
15) Isolation of Cellulose Decomposing Bacteria
16) Isolation of Starch Hydrolyzing Bacteria
17) Plasmid DNA Isolation

a) Preparation of stock solution of MS
b) Preparation of hormone stocks solution
5) Initiation of callus culture

Initiation & maintains of callus from inter nodal explants of stem explants of any available plant material.
6) Seed germination on media
7) Shoot culture
Root culture
9) Screening of plants using PCR techniques
a) DNA isolation
b) PCR amplification
c) Agarose gel electrophoresis
d) Gel Analysis
10) Encapsulation of embryo

Course work will also include interactive lectures on the basics of Plant Tissue Culture as well as its commercial applications.

RECOMBINANT DNA TECHNOLOGY, MICROBIOLOGY, GENE THERAPHY, BIOINFORMATICS, IMMUNOTECHNOLOGY etc

TRAINING ON THESE SUBJECTS COULD BE DONE AT ANY TIME DURING March, 2012 TILL August,2012.

WINTER PROJECTS

We are also conducting Winter projects during the Winter session from November till March, 2012 on various subjects like -
Biotechnology,Immunotechnology, Microbiology, Industrial Biotechnology, Industrial Microbiology Bioinformatics, Recombinant DNA Technology, Cell Biology, Environment Biotechnology, Plant Tissue Culture etc

Course Syllabus:
• Literature.
• Sequences file format and conversion tools.
• Different biological (nucleotide, proteomic) database with its applications.
• Sequence analysis tools – Blast, Fasta, PSI-Blast and RPS Blast.
• Protein information tools-PIR, Swis-Prot and ExPASy.
• Protein 3D structure and classification databases.
• Sequence alignments.
• Gene prediction methods – Principals and challenges.
• Molecular Phylogeny.
• Molecular Viewer.
• Drug Designing – Basic
• Protein Structure and Modeling
• Bioinformatics in computer aided drug design.
• Simulation and statistical protocol.
• Biochemical networks.
• Commercial bio-software.

1) Plasmid DNA isolation
2) Transformation
3) Restriction Digestion
4) PCR Amplification
5) Bioinformatics Basic – Theory
6) SDS Page
7) Replica Platting
IMVIC Test
9) Immuno Electrophoresis

and many more guest lectures…………

COURSE SYLLABUS

Basic Theory on Gene therapy

Isolation of Plant DNA, CTAB method

Isolation of Plasmid DNA

Quantitative analysis of DNA

Restriction Digestion techniques

Southern Blotting techniques

PCR techniques

Gene cloning and expression

Computational biology tools: Sequence and structure ret rival

Similarity search – BLAST / FASTA

Genomics Analysis tools

Gene identification and prediction: GENSCAN

Different Gene Delivery Method

Identification of the gene that plays the key role in the development of    the genetic disorder

Determination of the role of its product in health & disease

Venue :

SHRM Biotechnologies Pvt.Ltd. ,
Humaipur,P.O : Abdalpur, Madhyamgram ,
Kolkata-700155, (West Bengal , India)

Practical :

• Sterility Techniques & Preparation of Media
• Isolation of Micro – organism from Natural sources
• Staining Techniques
• Biochemical Tests
• Bacterial Growth Curve
• Pure Culture Isolation Technique
• Screening of Micro Organism for Industrial Applications
• Antibiotic Assays
• Quality Control of Milk
• Water Analysis
• Preparation of Yogurt
• Microbial Testing and Sterility testing of Finished Products

Theory :

• Microbial growth & Preservation
• Improvement of Industrial Micro Organism by Recombinant method
• Mutant Synthesizing improved level of metabolites
• Microbial Testing & Sterility Test
• Recent Trends of Industrial Microbiology

If interested , please contact us at:
Our contact no : (033) 64511686 / (033)2526-8988
Mail us at : info@shrmbio.com
Payment mode : Cash / DD ( in favour of SHRM Biotechnologies Pvt . Ltd , payable at Kolkata )

Venue :

SHRM Biotechnologies Pvt.Ltd. ,
Humaipur,P.O : Abdalpur, Madhyamgram ,
Kolkata-700155 ,(West Bengal , India)

Theory & computational Biology based Work :

• Introduction to NCBI Web sites
• Introduction to Databases
• Introduction to Genomics-information flow in biology,DNA sequence data,Experimental approach to Genome sequence data,Genome information resources.
• Improvement Of Industrial micro-organism by mutation & recombinant Method.
• Functional Proteomics-Protein sequence & structural dataProtein information resources and secondary databases .
• Computational Genomics-Internet basics,biological data analysis & application,sequence databases,NCBI model,file format.
• Sequence alignment & database search-Protein primary sequence analysis,DNA sequence analysis,pair wise sequence alignment,FASTA algorithm,BLAST,multiple sequence alignment,Database searching using BLAST and FASTA.

Practical

• DNA isolation-from plant cell(leaf of cabbage/mustard)/Animal cell (Goat Liver)/Human Blood(Fresh/Stored/Frozen) & Microbes .
• Plasmid DNA isolation.
• Gel Electrophoresis & Gel documentation & Photography
• Polymerase Chain reaction
• Short term Biochemical tests for Genetic toxicity .
• Bacterial Conjugation.
• DNA Fingerprinting

.

• Special theory session – Stem cell technology, Gene Therapy.
• Practical based assignments.

If interested , please contact us at:
Our contact no : (033) 64511686 / (033)2526-8988
Mail us at : info@shrmbio.com
Payment mode : Cash / DD ( in favour of SHRM Biotechnologies Pvt . Ltd , payable at Kolkata )

* Introduction To Microbiology.

* Commercial Importance.

* Media Optimization.

* Improvement Of Industrial micro-organism by mutation & recombinant Method.

* Regulatory Aspects.

* Bacterial recombination.

* Regulation.

* Microbial Expression Systems.

* Control Of Micro-organisms.

* Microbial Testing and Sterility Testing of Raw Materials and Finished Products.

* Application Of Microbiology in Commercial products.

Practical :

* Preparation of media for recombinant clones and wild type strains .

* Sterilization of media.

* Pure culture isolation.Pure plate,spread plate and streak plates.

* Preservation of wild type and recombinant cultures.

* Effect of Inoculum size on growth .

* Microscopic measurements,micrometer (ocular and stage),haemocytometer.

* Microscopic study of phytoplanktons & zooplanktons.

* Identification of Micro-organisms from the habitats
[Simple staining,differential staining,acid fast staining,capsule staining,spore staining and motility].

* Mutagenic treatment of bacterial culture.

* Antibiotic sensitivity test for bacterial cultures.

* Environmental monitoring.

* Isolation and enumeration of phosphate solubilising ,Cellulose decomposing and starch hydrolising bacteria from different habitats
(plate count method).

* Determination of potability of water following MPN method.Presumptive and confirmatory tests of coliforms.

* Isolation of symbiotic N2-fixing bacteria from root nodulesfrom leguminous.

* Isolation of Plasmid DNA from recombinant cultures.

Practical based assignments.

If interested , please contact us at:
Our contact no : (033) 64511686 / (033)2526-8988
Mail us at : info@shrmbio.com
Payment mode : Cash / DD (in favor of SHRM Biotechnologies Pvt. Ltd , payable at Kolkata )